2h
General HA(Histamine) ELISA Kit
General HA(Histamine) ELISA Kit
Assay length
Standard
400ng/mL
Sensitivity
1.81ng/mL
Detection range
4.94-400ng/mL
Assay Type
Competitive Inhibition
Alternative Names
Hyaluronan; Hyaluronate
Research Area
Tumor immunity;Hepatology;
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Hyaluronic Acid (HA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Hyaluronic Acid (HA) and unlabeled Hyaluronic Acid (HA) (Standards or samples) with the pre-coated antibody specific to Hyaluronic Acid (HA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Hyaluronic Acid (HA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Hyaluronic Acid (HA) in the sample.