General VD3(Vitamin D3) ELISA Kit

 

General VD3(Vitamin D3) ELISA Kit

Size

96T

Catalog no.

ELK8102

Price

526 EUR

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Assay length

2h

Standard

400ng/mL

Sensitivity

2.14ng/mL

Detection range

4.94-400ng/mL

Assay Type

Competitive Inhibition

Alternative Names

Cholecalciferol; Toxiferol; Activated 7-Dehydrocholesterol

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Research Area

Metabolic pathway;Reproductive science;Nutrition metabolism;Bone metabolism;

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Vitamin D3 (VD3) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Vitamin D3 (VD3) and unlabeled Vitamin D3 (VD3) (Standards or samples) with the pre-coated antibody specific to Vitamin D3 (VD3). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Vitamin D3 (VD3) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Vitamin D3 (VD3) in the sample.