General LPA(Lysophosphatidic Acid) ELISA Kit

2h

52.7ng/mL

10000ng/mL

123.5-10000ng/mL

Competitive Inhibition

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

LysoPA; 1-Oleoyl-sn-Glycero-3-Phosphate; Lysophosphatidyl Acid

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to LPA has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled LPA analogues and unlabeled antigen (Standards or samples) with the pre-coated antibody. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of LPA in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of LPA in the sample.