General cAMP(Cyclic Adenosine Monophosphate) ELISA Kit

2h

92.5pg/mL

20000pg/mL

246.9-20000pg/mL

Competitive Inhibition

Signal transduction;Metabolic pathway;

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

c-AMP; 3'-5'-Cyclic Adenosine Monophosphate; Adenosine Cyclophosphate

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Cyclic Adenosine Monophosphate (cAMP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Cyclic Adenosine Monophosphate (cAMP) and unlabeled Cyclic Adenosine Monophosphate (cAMP) (Standards or samples) with the pre-coated antibody specific to Cyclic Adenosine Monophosphate (cAMP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Cyclic Adenosine Monophosphate (cAMP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Cyclic Adenosine Monophosphate (cAMP) in the sample.