2h
General VC(Vitamin C) ELISA Kit
General VC(Vitamin C) ELISA Kit
Assay length
Standard
40000ng/mL
Sensitivity
192.1ng/mL
Detection range
493.83-40000ng/mL
Assay Type
Competitive Inhibition
Alternative Names
AA; L-Ascorbic Acid; Ascorbate
Research Area
Metabolic pathway;Infection immunity;Nutrition metabolism;
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Vitamin C (VC) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Vitamin C (VC) and unlabeled Vitamin C (VC) (Standards or samples) with the pre-coated antibody specific to Vitamin C (VC). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Vitamin C (VC) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Vitamin C (VC) in the sample.