General 12-HETE(12-Hydroxyeicosatetraenoic Acid) ELISA Kit

2h

12HETE

1000ng/mL

5.32ng/mL

12.35-1000ng/mL

Competitive Inhibition

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology;

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to 12-Hydroxyeicosatetraenoic Acid (12-HETE) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled 12-Hydroxyeicosatetraenoic Acid (12-HETE) and unlabeled 12-Hydroxyeicosatetraenoic Acid (12-HETE) (Standards or samples) with the pre-coated antibody specific to 12-Hydroxyeicosatetraenoic Acid (12-HETE). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of 12-Hydroxyeicosatetraenoic Acid (12-HETE) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of 12-Hydroxyeicosatetraenoic Acid (12-HETE) in the sample.