Cattle HB(Hemoglobin) ELISA Kit

 

Cattle HB(Hemoglobin) ELISA Kit

Size

96T

Catalog no.

ELK8165

Price

738 EUR

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Assay length

2h

Standard

1000ug/mL

Sensitivity

5.57ug/mL

Detection range

12.35-1000ug/mL

Assay Type

Competitive Inhibition

Alternative Names

Hgb; Haemoglobin; Heterotetramer(αβ)2

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Research Area

Metabolic pathway;Infection immunity;Hematology;Neuro science;

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Hemoglobin (HB) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Hemoglobin (HB) and unlabeled Hemoglobin (HB) (Standards or samples) with the pre-coated antibody specific to Hemoglobin (HB). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Hemoglobin (HB) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Hemoglobin (HB) in the sample.