Rat PTHrP(Parathyroid Hormone Related Protein) ELISA Kit

3h

6.2pg/mL

Sandwich

1000pg/mL

15.6-1000pg/mL

Rattus norvegicus

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Tumor immunity;Endocrinology;Hormone metabolism;Bone metabolism;

PTHLH; HHM; PTHR; PLP; Osteostatin; Parathyroid Hormone Like Hormone

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Hormone releasing factors and releasing hormones are  signaling molecules produced by glands in multicellular organisms. The glands that secrete Luteinizing hormones LHRG and LH, FSH comprise the endocrine signaling system. The term growth hormone releasing hormone GHRH is sometimes extended to include chemicals produced by cells that affect the same cell (autocrine or intracrine signaling) or nearby cells (paracrine signaling). Human recombinant LHRG and GHRH are produced in E. coli or in yeast cells.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Parathyroid Hormone Related Protein (PTHrP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Parathyroid Hormone Related Protein (PTHrP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Parathyroid Hormone Related Protein (PTHrP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Parathyroid Hormone Related Protein (PTHrP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.