2h
General ALCAR(Acetylcarnitine) ELISA Kit
General ALCAR(Acetylcarnitine) ELISA Kit
Assay length
Standard
5000ng/mL
Sensitivity
25.2ng/mL
Detection range
61.7-5000ng/mL
Alternative Names
Acetyl-L-carnitine
Assay Type
Competitive Inhibition
Research Area
Metabolic pathway;Neuro science;Nutrition metabolism;
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Acetylcarnitine (ALCAR) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Acetylcarnitine (ALCAR) and unlabeled Acetylcarnitine (ALCAR) (Standards or samples) with the pre-coated antibody specific to Acetylcarnitine (ALCAR). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Acetylcarnitine (ALCAR) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Acetylcarnitine (ALCAR) in the sample.