Guinea pig BDNF(Brain Derived Neurotrophic Factor) ELISA Kit

 

Guinea pig BDNF(Brain Derived Neurotrophic Factor) ELISA Kit

Size

96T

Catalog no.

ELK8219

Price

738 EUR

Buy at gentaur.com
Assay length

3h

Tissue

brain

Standard

10ng/mL

Assay Type

Sandwich

Sensitivity

0.059ng/mL

Detection range

0.156-10ng/mL

Alternative Names

Neurotrophin; Abrineurin

Research Area

Cytokine;Infection immunity;Neuro science;

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

About

Pigs and the smaller guinea pigs are frequent used as models for humans.

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Description

Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.Guinea pig ELISA kits for plasma and sera samples are used to study human genes through the guinea pig model (Cavia porcellus), also called the cavy rodent model. After mouzes and rats Guinea pigs are easy in maintained laboratory animals. cDNAs of Guinea pigs are also very popular.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Brain Derived Neurotrophic Factor (BDNF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Brain Derived Neurotrophic Factor (BDNF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Brain Derived Neurotrophic Factor (BDNF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Brain Derived Neurotrophic Factor (BDNF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.