Horse COL3(Collagen Type III) ELISA Kit

2h

1000ng/mL

4.98ng/mL

12.35-1000ng/mL

Competitive Inhibition

Metabolic pathway;Cardiovascular biology;

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Collagen Types in bones and muscles can be hydrolyzed and used in human grafts. ( Type II )

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Horse (Equus ferus caballus) sera and plasma contain equine IgGs, Immunoglobulins. ELISA test are used to determine quantitatively the presence in horse serum of the antigen by a polyclonal antibody to the equine epitope selected for the ELISA kit. A blocking solution for the native horse or equine immunoglobulins in available in the ELISA protocol.

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Collagen Type III (COL3) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Collagen Type III (COL3) and unlabeled Collagen Type III (COL3) (Standards or samples) with the pre-coated antibody specific to Collagen Type III (COL3). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Collagen Type III (COL3) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Collagen Type III (COL3) in the sample.