General CNT(Carnitine) ELISA Kit

 

General CNT(Carnitine) ELISA Kit

Size

96T

Catalog no.

ELK8228

Price

526 EUR

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Assay length

2h

Standard

1000ng/mL

Sensitivity

4.84ng/mL

Detection range

12.35-1000ng/mL

Alternative Names

VB20; Vitamin B20

Assay Type

Competitive Inhibition

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Carnitine (CNT) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Carnitine (CNT) and unlabeled Carnitine (CNT) (Standards or samples) with the pre-coated antibody specific to Carnitine (CNT). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Carnitine (CNT) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Carnitine (CNT) in the sample.