General VMA(Vanillylmandelic Acid) ELISA Kit

 

General VMA(Vanillylmandelic Acid) ELISA Kit

Size

96T

Catalog no.

ELK8239

Price

526 EUR

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Assay length

2h

Sensitivity

43.7ng/mL

Standard

10000ng/mL

Detection range

123.5-10000ng/mL

Assay Type

Competitive Inhibition

Alternative Names

Vanillyl Mandelic Acid

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Research Area

Metabolic pathway;Endocrinology;Cardiovascular biology;Hepatology;Gastroenterology;Hormone metabolism;

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Vanillylmandelic Acid (VMA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Vanillylmandelic Acid (VMA) and unlabeled Vanillylmandelic Acid (VMA) (Standards or samples) with the pre-coated antibody specific to Vanillylmandelic Acid (VMA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Vanillylmandelic Acid (VMA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Vanillylmandelic Acid (VMA) in the sample.