3h
Dog CALPRO(Calprotectin) ELISA Kit
Dog CALPRO(Calprotectin) ELISA Kit
Assay length
Standard
50ng/mL
Assay Type
Sandwich
Sensitivity
0.31ng/mL
Detection range
0.78-50ng/mL
Alternative Names
MRP-8/MRP-14; S100A8/A9
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Properties
Canine or Canis Lupus is mostly Beagle used for drug research. ELK Biotech produces ELISA test kits and polyclonal antibodies.E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test principle
The microplate provided in this kit has been pre-coated with an antibody specific to S100A8. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to S100A9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain calprotectin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of calprotectin in the samples is then determined by comparing the O.D. of the samples to the standard curve.