2h
General PIP3(Phosphatidylinositol Trisphosphate) ELISA Kit
General PIP3(Phosphatidylinositol Trisphosphate) ELISA Kit
Assay length
Standard
500ng/mL
Sensitivity
2.83ng/mL
Detection range
6.17-500ng/mL
Alternative Names
PtdIns(3,4,5)P3
Assay Type
Competitive Inhibition
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Phosphatidylinositol Trisphosphate (PIP3) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Phosphatidylinositol Trisphosphate (PIP3) and unlabeled Phosphatidylinositol Trisphosphate (PIP3) (Standards or samples) with the pre-coated antibody specific to Phosphatidylinositol Trisphosphate (PIP3). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Phosphatidylinositol Trisphosphate (PIP3) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Phosphatidylinositol Trisphosphate (PIP3) in the sample.