Pig Kim1(Kidney Injury Molecule 1) ELISA Kit

3h

kidney

Sandwich

2000pg/mL

12.1pg/mL

31.2-2000pg/mL

Tumor immunity;Infection immunity;Kidney biomarker;

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Pigs and the smaller guinea pigs are frequent used as models for humans.

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Whole adhesion and interacting molecules are  present in lysates used as reference for ELISA quantification of these molecules and their subunits.

HAVCR1; TIM1; TIMD1; HAVCR; Hepatitis A Virus Cellular Receptor 1; T Cell Immunoglobulin And Mucin Domain-Containing Protein 1; T-cell immunoglobulin mucin receptor 1

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kidney Injury Molecule 1 (Kim1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Kidney Injury Molecule 1 (Kim1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kidney Injury Molecule 1 (Kim1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Kidney Injury Molecule 1 (Kim1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.