General CS(Chondroitin Sulfate) ELISA Kit

2h

10000pg/mL

15.17pg/mL

39.06-10000pg/mL

Competitive Inhibition

Neuro science;Bone metabolism;

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Chondroitin Sulfate (CS) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Chondroitin Sulfate (CS) and unlabeled Chondroitin Sulfate (CS) (Standards or samples) with the pre-coated antibody specific to Chondroitin Sulfate (CS). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Chondroitin Sulfate (CS) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Chondroitin Sulfate (CS) in the sample.