3h
Sheep PRL(Prolactin) ELISA Kit
Sheep PRL(Prolactin) ELISA Kit
Assay length
Sensitivity
50pg/mL
Assay Type
Sandwich
Standard
8000pg/mL
Detection range
125-8000pg/mL
Alternative Names
LTH; Luteotropic Hormone
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Research Area
Endocrinology;Reproductive science;Genetic science;Hormone metabolism;
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prolactin (PRL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prolactin (PRL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prolactin (PRL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Prolactin (PRL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.