Pig FABP2(Intestinal Fatty Acid Binding Protein) ELISA Kit

3h

6.4pg/mL

Sandwich

1000pg/mL

15.6-1000pg/mL

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

FABPI; IFABP; I-FABP; Intestinal-type fatty acid-binding protein

Metabolic pathway;Tumor immunity;Endocrinology;Cardiovascular biology;

Pigs and the smaller guinea pigs are frequent used as models for humans.

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fatty Acid Binding Protein 2, Intestinal (FABP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fatty Acid Binding Protein 2, Intestinal (FABP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fatty Acid Binding Protein 2, Intestinal (FABP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fatty Acid Binding Protein 2, Intestinal (FABP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.