Rat NE(Noradrenaline/Norepinephrine) ELISA Kit

2h

8 pg/mL

Sandwich

400 pg/mL

Rattus norvegicus

12.5 pg/mL -200 pg/mL

NA; NAd; Norepinephrine; Norepi

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Signal transduction;Infection immunity;Neuro science;Hormone metabolism;

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Noradrenaline (NE) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Noradrenaline (NE) and unlabeled Noradrenaline (NE) (Standards or samples) with the pre-coated antibody specific to Noradrenaline (NE). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Noradrenaline (NE) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Noradrenaline (NE) in the sample.