T-Pro Phosphate-Buffered Saline (PBS, 10X) for western blot washing

Room Temperature

Room Temperature

Western Blot can be performed on tissues and cell extracts to determine the proteins present in your sample after PAGE electrophoresis and transfer on PVDF or Nitrocellulose. PVDF is more advised. The best detection method is using enhanced chemiluminescence or ECL.

Phosphate-buffered saline (abbreviated PBS) is a buffer solution and Phosphate Buffered Saline (PBS buffer), pH 7.4, stabilized is a water-based salt solution containing sodium hydrogen phosphate, sodium chloride and, in some formulations, potassium chloride and potassium dihydrogen phosphate.